With Brad Stanfield I agree. Whatever he is doing is not working as well as he would probably like.
I just looked him up. His skin looks alright no?
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Hi desertshores- wondering what form of Dasatnib you are using. The only one I have is tabs which Iâm thinking wouldnât be the best for dissolving into a skin formula. Do you have a powder? If so, source? ThanksâŚ
Much about what I am going to say was discussed in earlier threads, but in case you missed it:
Steps I use in making a dasatinib spray solution.
- Crush up your dasatinib tablets in a mortar and pestle. I use this one that I bought from Amazon.
-
Dissole dasatinib in 30ml Transcutol+DMSO. Allow the mixture to set awhile, stirring occasionally so that the dasatinib gets dissolved. There will some things in the tablet, like titanium dioxide that wonât dissolve. These will be filtered out later. Last batch I used 10ml DMSO and 20ml Transcutol.
-
Then add 70ml distilled water. (I actually just use bottled drinking water)
-
Mix and then filter. I use a small funnel and some coarse filter paper. (Yes, you could use coffee filter paper)
We just want to filter out any undissolved particulate that might clog our application sprayer.
- Put into small mist/spray bottles. Here are the ones I use, again from Amazon:
Disclaimer: I donât actually know the best concentration or the best ratios for the mixture. It is purely experimental and I am not recommending it to anyone.
âBased on the solubilizing properties of Transcutol and the poor water solubility of Dasatinib, it is very likely that Transcutol would be an effective solubilizing agent for Dasatinibâ
âDasatinib can be dissolved in DMSO at concentrations up to 200 mg/mLâ
You might add a little DMSO to your solution to ensure the dasatinib is fully dissolved
.
TranscutolÂŽ
(Diethylene Glycol
Monoethyl Ether): A Potential
Penetration Enhancer
https://sci-hub.se/10.1007/978-3-662-47039-8_12
Topical administration of the pan-Src kinase inhibitors,
dasatinib and LCB
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Oh, I meant Retin A. What is usually prescription strength? Thinking of ordering from India maybe.
I use 0.1% tretinoin.
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A new paper
Combined dasatinib and quercetin treatment contributes to skin rejuvenation through selective elimination of senescent cells in vitro and in vivo
The skinâs protective functions are compromised over time by both endogenous and exogenous aging. Senescence is well-documented in skin phenotypes, such as wrinkling and sagging, a consequence of the senescence-associated secretory phenotype (SASP) that involves the accumulation of senescent fibroblasts, chronic inflammation, and collagen remodeling. Although therapeutic approaches for eliminating senescent cells from the skin are available, their efficacy remains unclear. Accordingly, we aimed to examine the effects of dasatinib in combination with quercetin (D + Q) on senescent human skin fibroblasts and aging human skin. Senescence was induced in human dermal fibroblasts (HDFs) using approaches such as long-term passaging, ionizing radiation, and doxorubicin treatment. The generated senescent cells were treated with D + Q or vehicle. Additionally, a mouse-human chimera model was generated by subcutaneously transplanting whole-skin grafts of aged individuals onto nude mice. Mouse models were administered D + Q or vehicle by oral gavage for 30 days. Subsequently, skin samples were harvested and stained for senescence-associated beta-galactosidase. Senescence-associated markers were assessed by western blotting, reverse transcription-quantitative PCR and histological analyses. Herein, D + Q selectively eliminated senescent HDFs in all cellular models of induced senescence. Additionally, D + Q-treated aged human skin grafts exhibited increased collagen density and suppression of the SASP compared with control grafts. No adverse events were observed during the study period. Collectively, D + Q could ameliorate skin aging through selective elimination of senescent dermal fibroblasts and suppression of the SASP. Our findings suggest that D + Q could be developed as an effective therapeutic approach for combating skin aging.
paywalled
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